Tested negative via western blot for both hsv1 and 2. Anyway tldr: tested high positive via igg for hsv-2 value of 7.21. Selectively raising the ELISA index value defining HSV-2 positivity from >1.1 to >or=3.0 either among HSV-1-positive men or among those without a history or clinical evidence of genital lesions increased the PPV to >or=93%. There are so many things that could have happened to me as a result of this false positive and it’s inexcusable that these tests are being used even though they’re so clearly unreliable. Comparison of the Bio-Rad BioPlex HSV-1 IgG assay to the HerpeSelect HSV-1 EIA using prospective serum specimens (n505). The results are summarized in Tables 1 and 2 below: Table 1. Western blot positivity was more common in men without herpes simplex virus type 1 (HSV-1) antibody than in those with HSV-1 antibody (93% vs 76%, P = 0.02) and in men with a history or clinical evidence of genital lesions (88% vs 80%, P = 0.30). Further discrepancies were evaluated by glycoprotein G type-specific Western blot (WB) at the University of Washington Virology laboratory. The western blot is neither quick nor widely available, but can be simply. Women who were HSV-2 seropositive but had no reported or recognized recurrences were followed.
Ninety-one (84%) of 108 HSV-2 ELISA-positive sera tested HSV-2 Western blot-positive. The ELISA tests have excellent ability to detect herpes antibodies when they. underwent a detailed interview and an HSV serological test using Western blot assay. HSV-2 Western blots were then prospectively performed on sequential sera with indeterminate HSV-2 ELISAs. I would go to a primary care doctor that can refer you. HSV-2 Western blots were performed on sera from male sexually transmitted disease clinic patients testing HSV-2 ELISA-positive and used to define a new class of indeterminate HSV-2 ELISA result. Love, Quest definitely won’t help you with drawing for the Western Blot since they own the patent to the cheaper, less accurate Hsv test. The objective of this study was to define the positive predictive value (PPV) of the Focus herpes simplex virus type 2 (HSV-2) enzyme-linked immunosorbent assay (ELISA) in a low HSV-2 prevalence population and to develop a new test interpretation algorithm.
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